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Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry1Ab protein Lane 3: 2 µg of Cry1Ab protein
Cry1Ab
Purity
> 91 % by SDS-PAGE
Molecular Weight (MW)
130.62 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
1,155 AA (1-1,155)
Purification methods
Inclusion body purification and solubilization, anion exchange and size exclusion chromatography
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 10 % Glycerol
Additional information
N/A
Sequence
MDNNPNINECIPYNCLSNPEVEVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFLVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREWEADPTNPALREEMRIQFNDMNSALTTAIPLFAVQNYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDHAVRWYNTGLERVWGPDSRDWIRYNQFRRELTLTVLDIVSLFPNYDSRTYPIRTVSQLTREIYTNPVLENFDGSFRGSAQGIEGSIRSPHLMDILNSITIYTDAHRGEYYWSGHQIMASPVGFSGPEFTFPLYGTMGNAAPQQRIVAQLGQGVYRTLSSTLYRRPFNIGINNQQLSVLDGTEFAYGTSSNLPSAVYRKSGTVDSLDEIPPQNNNVPPRQGFSHRLSHVSMFRSGFSNSSVSIIRAPMFSWIHRSAEFNNIIPSSQITQIPLTKSTNLGSGTSVVKGPGFTGGDILRRTSPGQISTLRVNITAPLSQRYRVRIRYASTTNLQFHTSIDGRPINQGNFSATMSSGSNLQSGSFRTVGFTTPFNFSNGSSVFTLSAHVFNSGNEVYIDRIEFVPAEVTFEAEYDLERAQKAVNELFTSSNQIGLKTDVTDYHIDQVSNLVECLSDEFCLDEKKELSEKVKHAKRLSDERNLLQDPNFRGINRQLDRGWRGSTDITIQGGDDVFKENYVTLLGTFDECYPTYLYQKIDESKLKAYTRYQLRGYIEDSQDLEIYLIRYNAKHETVNVPGTGSLWPLSAPSPIGKCAHHSHHFSLDIDVGCTDLNEDLGVWVIFKIKTQDGHARLGNLEFLEEKPLVGEALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDALFVNSQYDRLQADTNIAMIHAADKRVHSIREAYLPELSVIPGVNAAIFEELEGRIFTAFSLYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNHRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEVYPNNTVTCNDYTATQEEYEGTYTSRNRGYDGAYESNSSVPADYASAYEEKAYTDGRRDNPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry1Ac protein Lane 3: 2 µg of Cry1Ac protein
Cry1Ac
Purity
> 93 % by SDS-PAGE
Molecular Weight (MW)
133.33 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
1,178 AA (1-1,178)
Purification methods
Inclusion body purification and solubilization, anion exchange and buffer exchange
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 10 % Glycerol
Additional information
N/A
Sequence
MDNNPNINECIPYNCLSNPEVEVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFLVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREWEADPTNPALREEMRIQFNDMNSALTTAIPLFAVQNYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDYAVRWYNTGLERVWGPDSRDWVRYNQFRRELTLTVLDIVALFPNYDSRRYPIRTVSQLTREIYTNPVLENFDGSFRGSAQGIERSIRSPHLMDILNSITIYTDAHRGYYYWSGHQIMASPVGFSGPEFTFPLYGTMGNAAPQQRIVAQLGQGVYRTLSSTLYRRPFNIGINNQQLSVLDGTEFAYGTSSNLPSAVYRKSGTVDSLDEIPPQNNNVPPRQGFSHRLSHVSMFRSGFSNSSVSIIRAPMFSWIHRSAEFNNIIASDSITQIPAVKGNFLFNGSVISGPGFTGGDLVRLNSSGNNIQNRGYIEVPIHFPSTSTRYRVRVRYASVTPIHLNVNWGNSSIFSNTVPATATSLDNLQSSDFGYFESANAFTSSLGNIVGVRNFSGTAGVIIDRFEFIPVTATLEAEYNLERAQKAVNALFTSTNQLGLKTNVTDYHIDQVSNLVTYLSDEFCLDEKRELSEKVKHAKRLSDERNLLQDSNFKDINRQPERGWGGSTGITIQGGDDVFKENYVTLSGTFDECYPTYLYQKIDESKLKAFTRYQLRGYIEDSQDLEIYLIRYNAKHETVNVPGTGSLWPLSAQSPIGKCGEPNRCAPHLEWNPDLDCSCRDGEKCAHHSHHFSLDIDVGCTDLNEDLGVWVIFKIKTQDGHARLGNLEFLEEKPLVGEALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDALFVNSQYDQLQADTNIAMIHAADKRVHSIREAYLPELSVIPGVNAAIFEELEGRIFTAFSLYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNQRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEIYPNNTVTCNDYTVNQEEYGGAYTSRNRGYNEAPSVPADYASVYEEKSYTDGRRENPCEFNRGYRDYTPLPVGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry1Da1 protein Lane 3: 2 µg of Cry1Da1 protein
Cry1Da1
Purity
> 96 % by SDS-PAGE
Molecular Weight (MW)
132.48 kDa
Aliquot size
1 mL
Concentration
1 mg/mL & 2 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
1,165 AA (1-1,165)
Purification methods
Inclusion body purification and solubilization, anion exchange and buffer exchange
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 10 % Glycerol
Additional information
N/A
Sequence
MEINNQNQCVPYNCLSNPKEIILGEERLETGNTVADISLGLINFLYSNFVPGGGFIVGLLELIWGFIGPSQWDIFLAQIEQLISQRIEEFARNQAISRLEGLSNLYKVYVRAFSDWEKDPTNPALREEMRIQFNDMNSALITAIPLFRVQNYEVALLSVYVQAANLHLSILRDVSVFGERWGYDTATINNRYSDLTSLIHVYTNHCVDTYNQGLRRLEGRFLSDWIVYNRFRRQLTISVLDIVAFFPNYDIRTYPIQTATQLTREVYLDLPFINENLSPAASYPTFSAAESAIIRSPHLVDFLNSFTIYTDSLARYAYWGGHLVNSFRTGTTTNLIRSPLYGREGNTERPVTITASPSVPIFRTLSYITGLDNSNPVAGIEGVEFQNTISRSIYRKSGPIDSFSELPPQDASVSPAIGYSHRLCHATFLERISGPRIAGTVFSWTHRSASPTNEVSPSRITQIPWVKAHTLASGASVIKGPGFTGGDILTRNSMGELGTLRVTFTGRLPQSYYIRFRYASVANRSGTFRYSQPPSYGISFPKTMDAGEPLTSRSFAHTTLFTPITFSRAQEEFDLYIQSGVYIDRIEFIPVTATFEAEYDLERAQKVVNALFTSTNQLGLKTDVTDYHIDQVSNLVACLSDEFCLDEKRELSEKVKHAKRLSDERNLLQDPNFRGINRQPDRGWRGSTDITIQGGDDVFKENYVTLPGTFDECYPTYLYQKIDESKLKAYTRYQLRGYIEDSQDLEIYLIRYNAKHEIVNVPGTGSLWPLSVENQIGPCGEPNRCAPHLEWNPDLHCSCRDGEKCAHHSHHFSLDIDVGCTDLNEDLGVWVIFKIKTQDGHARLGNLEFLEEKPLLGEALARVKRAEKKWRDKRETLQLETTIVYKEAKESVDALFVNSQYDRLQADTNIAMIHAADKRVHRIREAYLPELSVIPGVNAAIFEELEERIFTAFSLYDARNIIKNGDFNNGLLCWNVKGHVEVEEQNNHRSVLVIPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFNNCVEEEVYPNNTVTCINYTATQEEYEGTYTSRNRGYDEAYGNNPSVPADYASVYEEKSYTDRRRENPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry1Fa protein Lane 3: 2 µg of Cry1Fa protein
Cry1Fa
Purity
> 91 % by SDS-PAGE
Molecular Weight (MW)
133.62 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
1,174 AA (1-1,174)
Purification methods
Inclusion body purification and solubilization, and buffer exchange
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 1 % Trehalose
Additional information
N/A
Sequence
MENNIQNQCVPYNCLNNPEVEILNEERSTGRLPLDISLSLTRFLLSEFVPGVGVAFGLFDLIWGFITPSDWSLFLLQIEQLIEQRIETLERNRAITTLRGLADSYEIYIEALREWEANPNNAQLREDVRIRFANTDDALITAINNFTLTSFEIPLLSVYVQAANLHLSLLRDAVSFGQGWGLDIATVNNHYNRLINLIHRYTKHCLDTYNQGLENLRGTNTRQWARFNQFRRDLTLTVLDIVALFPNYDVRTYPIQTSSQLTREIYTSSVIEDSPVSANIPNGFNRAEFGVRPPHLMDFMNSLFVTAETVRSQTVWGGHLVSSRNTAGNRINFPSYGVFNPGGAIWIADEDPRPFYRTLSDPVFVRGGFGNPHYVLGLRGVAFQQTGTNHTRTFRNSGTIDSLDEIPPQDNSGAPWNDYSHVLNHVTFVRWPGEISGSDSWRAPMFSWTHRSATPTNTIDPERITQIPLVKAHTLQSGTTVVRGPGFTGGDILRRTSGGPFAYTIVNINGQLPQRYRARIRYASTTNLRIYVTVAGERIFAGQFNKTMDTGDPLTFQSFSYATINTAFTFPMSQSSFTVGADTFSSGNEVYIDRFELIPVTATFEAEYDLERAQKAVNALFTSINQIGIKTDVTDYHIDQVSNLVDCLSDEFCLDEKRELSEKVKHAKRLSDERNLLQDPNFKGINRQLDRGWRGSTDITIQRGDDVFKENYVTLPGTFDECYPTYLYQKIDESKLKPYTRYQLRGYIEDSQDLEIYLIRYNAKHETVNVLGTGSLWPLSVQSPIRKCGEPNRCAPHLEWNPDLDCSCRDGEKCAHHSHHFSLDIDVGCTDLNEDLDVWVIFKIKTQDGHARLGNLEFLEEKPLVGEALARVKRAEKKWRDKREKLELETNIVYKEAKESVDALFVNSQYDQLQADTNIAMIHAADKRVHRIREAYLPELSVIPGVNVDIFEELKGRIFTAFFLYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNHRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEVYPNNTVTCNDYTANQEEYGGAYTSRNRGYDETYGSNSSVPADYASVYEEKSYTDGRRDNPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry1Ie2 protein Lane 3: 2 µg of Cry1Ie2 protein
Cry1Ie2
Purity
> 97 % by SDS-PAGE
Molecular Weight (MW)
81.49 kDa
Aliquot size
1 mL
Concentration
1 mg/mL & 2 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
719 AA (1-719)
Purification methods
Inclusion body purification and solubilization, anion exchange and size exclusion chromatography
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 10 % Glycerol
Additional information
N/A
Sequence
MKSKNQDMYQRLSYNTTVDKNSTDSLRNETDIELKNINHEDFLRMSEHESIDPFVNVSTIQTGIGIAGKILGTLGVPFAGQIASLYSFILGELWPKGKSQWEIFMEHVEELIDQKISTYARNIALADLKGLGDALAVYHESLESWIKNRNNARATSVVKSQYIALELLFVQKLPSFAVSGEEVPLLPIYAQAANLHLLLLRDASVFGKEWGLSNSQISTFYNRQVERTSDYSDHCVKWYSTGLNNLRGTNAESWVRYNQFRKDMTLMVLDLIALFPSYDTLVYPIKTTSQLTREVYTDAIGTVHPNASFASTTWYNNNAPSFSAIESAVVRNPHLLDFLEQVTIYSLLSRWSNTQYMNMWGGHRLEFRTIGGVLNTSTQGSTNTSINPVTLPFTSRDVYRTESLAGLNLFLTQPVNGVPRVDFHWKFATLPIASDNFYYPGYAGIGTQLQDSENELPPETTGQPNYESYSHRLSHIGLISASHVKALVYSWTHRSADRTNTIEPNSITQIPLVKAFNLSSGAAVVRGPGFTGGDILRRTNTGTFGDIRVNINPPFAQRYRVRIRYASTTDLQFHTSINGRAINQGNFSATMNRGEDLEYRTFRTVGFTTPFSFSDVQSTFTIGAWNFSSGNDVYIDRIEFVPVEVPYEEEYDFEKVQEEVTALFTSTNPRELKTDVTDYHIDQVSNLVESLSDEFYLDEKRELFEIVKYVKQLNIERDM*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Cry2Ab2 protein Lane 3: 2 µg of Cry2Ab2 protein
Cry2Ab2
Purity
> 93 % by SDS-PAGE
Molecular Weight (MW)
70.74 kDa
Aliquot size
1 mL
Concentration
1 mg/mL & 2 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
633 AA (1-633)
Purification methods
Inclusion body purification and solubilization, anion exchange and size exclusion chromatography
Species
Bacillus thuringiensis
Storage buffer
10 mM CAPS pH 11.0, 10 % Glycerol
Additional information
N/A
Sequence
MNSVLNSGRTTICDAYNVAAHDPFSFQHKSLDTVQKEWTEWKKNNHSLYLDPIVGTVASFLLKKVGSLVGKRILSELRNLIFPSGSTNLMQDILRETEKFLNQRLNTDTLARVNAELTGLQANVEEFNRQVDNFLNPNRNAVPLSITSSVNTMQQLFLNRLPQFQMQGYQLLLLPLFAQAANLHLSFIRDVILNADEWGISAATLRTYRDYLKNYTRDYSNYCINTYQSAFKGLNTRLHDMLEFRTYMFLNVFEYVSIWSLFKYQSLLVSSGANLYASGSGPQQTQSFTSQDWPFLYSLFQVNSNYVLNGFSGARLSNTFPNIVGLPGSTTTHALLAARVNYSGGISSGDIGASPFNQNFNCSTFLPPLLTPFVRSWLDSGSDREGVATVTNWQTESFETTLGLRSGAFTARGNSNYFPDYFIRNISGVPLVVRNEDLRRPLHYNEIRNIASPSGTPGGARAYMVSVHNRKNNIHAVHENGSMIHLAPNDYTGFTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQNNTTARYTLRGNGNSYNLYLRVSSIGNSTIRVTINGRVYTATNVNTTTNNDGVNDNGARFSDINIGNVVASSNSDVPLDINVTLNSGTQFDLMNIMLVPTNISPLY*
EPSPS (5-enolpyruvylshikimate-3-phosphate synthase)
Purity
TBD by SDS-PAGE
Molecular Weight (MW)
47.58 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
455 AA (1-455)
Purification methods
TBD
Species
Agrobacterium sp.
Storage buffer
TBD
Additional information
N/A
Sequence
MSHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPTPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADGVRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTLQEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEGATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVATHLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS G12C protein Lane 3: 2 µg of Human His-KRAS G12C protein
Human His-KRAS G12C
Purity
> 99 % by SDS-PAGE
Molecular Weight (MW)
21.9 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12C
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
192 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
40 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag is intact with TEV site at N-terminal
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS G12D protein Lane 3: Molecular weight marker Lane 4: 2 µg of Human His-KRAS G12D protein
Human His-KRAS G12D
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
21.8 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12D
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
191 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag intact
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS G12R protein Lane 3: Molecular weight marker Lane 4: 2 µg of Human His-KRAS G12R protein
Human His-KRAS G12R
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
21.8 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12R
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
191 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag intact
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGARGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS G12V protein Lane 3: 2 µg of Human His-KRAS G12V protein
Human His-KRAS G12V
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
21.9 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12V
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
191 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag intact
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS G13D protein Lane 3: 2 µg of Human His-KRAS G13D protein
Human His-KRAS G13D
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
21.9 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G13D
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
191 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag intact
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGAGDVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human His-KRAS WT protein Lane 3: 2 µg of Human His-KRAS WT protein
Human His-KRAS WT
Purity
> 95 % by SDS-PAGE
Molecular Weight (MW)
21.8 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
N-terminal His-tag
Storage condition
Frozen, – 80 °C
Protein length
192 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
40 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
His-tag is intact with TEV site at N-terminal
Sequence
MGSSHHHHHHHHGSSGENLYFQGMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS G12C protein Lane 3: 2 µg of Human KRAS G12C protein
Human KRAS G12C
Purity
> 99 % by SDS-PAGE
Molecular Weight (MW)
19.3 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12C
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
40 mM HEPES pH 8.0, 150 mM NaCl, 5 m MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS G12D protein Lane 3: Molecular weight marker Lane 4: 2 µg of Human KRAS G12D protein
Human KRAS G12D
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
19.4 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12D
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS G12R protein Lane 3: Molecular weight marker Lane 4: 2 µg of Human KRAS G12R protein
Human KRAS G12R
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
19.4 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12R
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGARGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS G12V protein Lane 3: 2 µg of Human KRAS G12V protein
Human KRAS G12V
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
19.3 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G12V
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS G13D protein Lane 3: 2 µg of Human KRAS G13D protein
Human KRAS G13D
Purity
> 98 % by SDS-PAGE
Molecular Weight (MW)
19.4 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
G13D
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGAGDVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Human KRAS WT protein Lane 3: 2 µg of Human KRAS WT protein
Human KRAS WT
Purity
> 99 % by SDS-PAGE
Molecular Weight (MW)
19.3 kDa
Aliquot size
200 µL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
170 AA (1-169)
Purification methods
Ni-NTA affinity and size exclusion chromatography
Species
Human
Storage buffer
40 mM HEPES pH 8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, 10 % Glycerol
Additional information
N-terminal His-tag was removed by TEV protease, a Glycine as a scar sequence
Sequence
GMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of PAT protein Lane 3: 2 µg of PAT protein
PAT (Phosphinothricin N-acetyltransferase)
Purity
> 78 % by SDS-PAGE
Molecular Weight (MW)
20.61 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
183 AA (1-183)
Purification methods
Anion exchange and size exclusion chromatography
Species
Streptomyces viridochromogenes
Storage buffer
10 mM Tris pH 7.5, 0.02 % Sodium azide
Additional information
N/A
Sequence
MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDFELPAPPRPVRPVTQI*
Figure. 4-12 % Bis-Tris SDS-PAGE Analysis Lane 1: Molecular weight marker Lane 2: 1 µg of Vip3Aa19 protein Lane 3: 2 µg of Vip3Aa19 protein
Vip3Aa19
Purity
> 93 % by SDS-PAGE
Molecular Weight (MW)
88.67 kDa
Aliquot size
1 mL
Concentration
1 mg/mL
Mutation
N/A
Form
Liquid
Expression system
Affinity tag
No Tag
Storage condition
Frozen, – 80 °C
Protein length
789 AA (1-789)
Purification methods
Anion exchange and buffer exchange
Species
Bacillus thuringiensis
Storage buffer
10 mM Tris pH 8.0, 10 % Glycerol (or 1 % Trehalose)
Additional information
N/A
Sequence
MNKNNTKLSTRALPSFIDYFNGIYGFATGIKDIMNMIFKTDTGGDLTLDEILKNQQLLNDISGKLDGVNGSLNDLIAQGNLNTELSKEILKIANEQNQVLNDVNNKLDAINTMLRVYLPKITSMLSDVMKQNYALSLQIEYLSKQLQEISDKLDIINVNVLINSTLTEITPAYQRIKYVNEKFEELTFATETSSKVKKDGSPADILDELTELTELAKSVTKNDVDGFEFYLNTFHDVMVGNNLFGRSALKTASELITKENVKTSGSEVGNVYNFLIVLTALQAQAFLTLTTCRKLLGLADIDYTSIMNEHLNKEKEEFRVNILPTLSNTFSNPNYAKVKGSDEDAKMIVEAKPGHALIGFEISNDSITVLKVYEAKLKQNYQVDKDSLSEVIYGDMDKLLCPDQSEQIYYTNNIVFPNEYVITKIDFTKKMKTLRYEVTANFYDSSTGEIDLNKKKVESSEAEYRTLSANDDGVYMPLGVISETFLTPINGFGLQADENSRLITLTCKSYLRELLLATDLSNKETKLIVPPSGFISNIVENGSIEEDNLEPWKANNKNAYVDHTGGVNGTKALYVHKDGGISQFIGDKLKPKTEYVIQYTVKGKPSIHLKDENTGYIHYEDTNNNLEDYQTINKRFTTGTDLKGVYLILKSQNGDEAWGDNFIILEISPSEKLLSPELINTNNWTSTGSTNISGNTLTLYQGGRGILKQNLQLDSFSTYRVYFSVSGDANVRIRNSREVLFEKRYMSGAKDVSEMFTTKFEKDNFYIELSQGNNLYGGPIVHFYDVSIK*