Protein Production
Helix's versatile recombinant protein production capabilities include gene construct design, protein expression, and protein purification. Proteins can be produced for any downstream analytical technique, from assays to biophysical and structural characterization, across both biopharmaceutical and agricultural applications. Helix's expertise includes crystallization-grade protein production with purity of 95% or higher.
Construct Design/ Synthesis
Protein design matters. We work with you to ensure each construct sequence is biologically relevant, has appropriate purification tags, and meets requirements for its intended assays or biophysical/structural characterization.
Pilot Expression Testing
Pilot testing proactively fine tunes expression and purification parameters before scale up, saving time and cost. We use small-scale cell culture to test the construct we've designed, looking not only at whether protein expression occurs, but also how much expression will ensure success during scale-up expression and purification. (Optional for clients having well-characterized expression and purification data)
Expression Optimization
When protein expression isn’t optimal, Helix works closely with clients to adjust parameters and increase yield. Techniques include testing under different expression conditions, changing the cell line, optimizing the construct, and/or using an optimal buffer condition that will maintain protein quality.
Scale-up Expression & Purification
Using what is identified during pilot expression testing (or using the client’s data), Helix will produce the amount of protein required for the experiment, from milligrams to grams.
Quantitative Analysis/Quality Control
Each recombinant protein produced includes rigorous quantitative analysis (QA) and quality control (QC). For QA/QC, Helix uses SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), chromatograms, and mass spectrometry to gauge the purity of the protein. Additional analyses of final product concentration include spectrophotometry (UV-Vis), densitometry, and, if required, biophysical characterization to further qualify the protein (i.e. thermal shift assay, DLS, mass photometry, peptide mass fingerprinting).
Never feel lost in the process again
Throughout the protein production process, project status is communicated weekly. The Helix protein production team provides detailed technical project reports at the end of each stage (time per stage varies depending on which expression system is used).
"We have consistently been impressed by Helix's expertise, quality, speed, and thoughtfulness throughout our work together. They have proven to be an ideal partner in our structural biology projects, consistently delivering impressive results and maintaining excellent communication every step of the way."
"Helix Biostructures has been delivering exceptional protein production and characterization services to us in the agricultural biotechnology sector. Our work together is defined by a shared dedication to excellence and an in-depth knowledge of the international regulations and standards necessary for the deregulation of biotechnological crops. Our collaboration makes a substantial contribution to our clients' endeavors in sustainably offering biotechnological solutions for crop protection and increased production."
"Collaborating with Helix to elucidate the structure of our therapeutic antibody in complex with its antigen through Cryo-EM was very valuable for our research. Their expertise in protein sciences and structural biology gave us detailed insights into the antibody-antigen allosteric mechanism, advancing our understanding significantly. The data they provided exceeded our expectations and contributed to the publication of a high-impact paper. The professionalism and technical skill of their team made the entire process smooth and efficient."
