Cryo-EM
High-throughput, Financially Accessible Cryo-EM
In cases where x-ray crystallography is unsuitable for structural characterization, including certain protein complexes, viruses, antibodies, membrane proteins, and ion channels, Helix offers flexible access to cryo-electron microscopy expertise and services using a milestone-based or by-the-sample approach.
High-throughput Grid Screening
Screening to determine optimal grid conditions
Precise grid preparation
Flexible "by-the-grid" pricing
High-resolution Data Collection
Data collection (with collection speeds of between 5,000 and 15,000 micrographs per day, depending on grid type and collection parameters)
Flexible "by-the-hour" pricing
Feasibility Study
Grid Optimization
Pilot Data Set
Initial 2D and 3D Classes
Cryo-EM Data Processing
Expertise to resolve flexible regions of protein and protein complexes
In-house cryo-EM data processing and analysis
Flexible "by-the-dataset" pricing
Protein-to-structure
In-house protein construct design, expression, and purification
High-resolution 3D structure from soluble protein
Milestone-based Cryo-EM Services
Grid Screening
Vitrify sample, capture small number of images representing thickness range, evaluate by eye for optimal particle mono-dispersity, density and morphology, and deliver report on recommended next steps.
Pilot dataset and processing
Collect ~500 micrograph movies, using the same conditions/setup as a full dataset; process data; and, if 2D processing goes well, proceed to 3D for deeper insight.
Full dataset collection and processing
to "Collect 5,000 to 15,000 micrograph movies over 24 hours (depending on grid type and collection parameters), and report on findings and expert analysis (final particle count, resolution, FSC curves, particle angular distribution, sharpening and local resolution). Also provide half maps, full map, sharpened map and sharpened map filters to local resolution.
Atomic model-building
Using either an initial model or directly interpreting the sequence from a high-resolution map, Helix experts deliver a comprehensive Table 1 summary detailing model geometry statistics and map-to-model fit. The final atomic model is provided in both .pdb and .cif formats, ensuring that your data is ready for publication or further analysis.
"We have consistently been impressed by Helix's expertise, quality, speed, and thoughtfulness throughout our work together. They have proven to be an ideal partner in our structural biology projects, consistently delivering impressive results and maintaining excellent communication every step of the way."
"Helix Biostructures has been delivering exceptional protein production and characterization services to us in the agricultural biotechnology sector. Our work together is defined by a shared dedication to excellence and an in-depth knowledge of the international regulations and standards necessary for the deregulation of biotechnological crops. Our collaboration makes a substantial contribution to our clients' endeavors in sustainably offering biotechnological solutions for crop protection and increased production."
"Collaborating with Helix to elucidate the structure of our therapeutic antibody in complex with its antigen through Cryo-EM was very valuable for our research. Their expertise in protein sciences and structural biology gave us detailed insights into the antibody-antigen allosteric mechanism, advancing our understanding significantly. The data they provided exceeded our expectations and contributed to the publication of a high-impact paper. The professionalism and technical skill of their team made the entire process smooth and efficient."
